cDNA Sequencing.

Wasatch BioLabs' PCR cDNA sequencing service, powered by Oxford Nanopore Technologies (ONT), offers an advanced method for sequencing full-length cDNA molecules. This approach provides researchers with detailed insights into gene expression, alternative splicing, and isoform diversity by capturing the complete structure of RNA transcripts. By sequencing the entire length of cDNA, this service enables a deeper understanding of transcriptomic landscapes, essential for unraveling the complexities of gene regulation and function.

The Power Of Long Read Sequencing

Explore the advantages of WBL’s Long-Read cDNA Sequencing compared short-read cDNA sequencing methods

Direct cDNA Sequencing:
Analysis Landscape

Quantitative Gene Expression Analysis: Measure the relative abundance of transcripts to determine gene expression levels across different conditions or time points.
Alternative Splicing Analysis: Identify and quantify different splice variants, enabling the study of isoform diversity and regulation.
Isoform-Level Expression Analysis: Analyze the expression of specific isoforms, providing insights into the functional diversity of genes.
Differential Gene Expression: Compare gene expression profiles between different samples or conditions to identify genes that are upregulated or downregulated.
Fusion Gene Detection: The platform’s flexibility allows for the development of custom assays tailored to specific research or clinical needs, which can be crucial for personalized medicine applications.
Single-Cell Gene Expression: When combined with single-cell techniques, cDNA sequencing can be used to study gene expression at the single-cell level, providing insights into cellular heterogeneity.

Direct RNA Sequencing:
Applications and Use Cases

WBL Supports cDNA Sequencing Genomes Of All Organisms:

Humans
Animals
Plants
Bacteria*
Virus*

Sample types accepted:

RNA

*WBL currently only accepts DNA and RNA that are extracted from samples that are not classified as infectious. This policy ensures the safety of our laboratory personnel and compliance with regulatory standards. Please ensure that all samples are appropriately treated and certified as non-infectious before submission. If you have any questions about sample preparation or our safety standards, please contact our support team at: support@wasatchbiolabs.com.

WBL: Your Competitive Edge

Maximize your sequencing output with WBL’s leading technology, streamlined bioinformatics pipelines, and expert guidance. WBL’s team of researchers are pioneers in Oxford Nanopore Technologies sequencing with decades of experience in genomics and bioinformatics research.

Robust Data

NGS 3.0 data with fully streamlined bioinformatics pipelines.

Fast Turnarounds

Results within 14-21 days of your sample’s arrival at the lab.

Expert Guidance

Teams of dedicated researchers and project managers to support your research and development.

Full Visibility

Direct access to an online portal for sample information and batch status at each step of the sequencing process.

Specialized offerings in our next-generation     biolab

Explore our other RNA Services