Direct Targeted Methylation Sequencing (gdna)

Native Methylation Meets Targeted Precision

Wasatch BioLabs' Direct Targeted Methylation Sequencing (dTMS) provides a scalable, cost-effective solution for region-specific analysis, ideal for applications where whole-genome sequencing is inefficient or expensive. Combining hybridization-based enrichment with nanopore sequencing, dTMS overcomes the limitations of traditional methods, including short-reads and synthetic DNA that erase native modifications.

dTMS enables the direct targeting of genes, regions, and epigenetic modifications, capturing complex sequences, and native methylation and hydroxymethylation with enhanced sensitivity and specificity.

Advantages

Direct, native detection bypasses the need for bisulfite conversion and PCR amplification, eliminating their associated biases and DNA damage. WBL's sequencing technology enables the analysis of:

  • Copy Number Variants (CNVs, dependent on read length and depth)
  • Modified Bases(5mC, 5hmC; also 6mA for bacteria)
  • Short Tandem Repeat Expansions (STRs, dependent on read length and depth)
  • Phasing Data (dependent on read length and depth)
  • Small Variants (SNPs)
  • Structural Variants

Targeted Bisulfite Sequencing

WBL dTMS (gDNA)

Long Reads

Single Molecule Resolution

Methylation & Hydroxy Methylation

Real-Time Analysis

PCR Bias-Free

Bisulfite Conversion DNA Damage-Free

DNA Sequence

Methylation

Hydroxymethylation

Potential For Additional
Base Modifications

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*Requires Extra Steps

Traditional dTMS   Vs   WBL dTMS

A Technical Comparrison

dTMS leverages proprietary library preparation and hybridization-based enrichment protocols to streamline the targeted acquisition of native sequence and methylation data. WBL’s protocol bypasses the amplification and conversion steps required in traditional methylation analyses, reducing errors and biases in your data.

The Setup Process

More Than a Service Provider —Your Trusted Partner

From exploratory research to targeted analyses, Wasatch BioLabs empowers precision analysis with unbiased sequencing solutions. Leveraging proprietary technologies like dTMS, tailored workflows, and expert bioinformatics support, we deliver actionable insights to advance your research.

Proprietary, Flexible Solutions

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Harness advanced tools like dTMS to target genes, complex variants, and epigenetic modifications. Our workflows scale from exploration to clinical applications.

Bioinformatic Expertise

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Enhance your data with custom bioinformatics solutions, using tailored workflows and advanced analysis to generate impactful, publication-ready insights.

One-On-One Collaboration

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Collaborate with sequencing and bioinformatics experts who provide personalized guidance, ensuring your project moves smoothly from biomarker discovery to targeted analysis.

Specialized offerings in our next-generation Biolab

Explore our other DNA Service