Bisulfite-Free Targeted Methylation Sequencing

Wasatch BioLabs’ exclusive technology enables targeted sequencing at thousands of regions of interest with enrichment of 400-2300x and depth that can be scaled according to researchers' needs. Unlike other traditional targeted sequencing assays, WBL’s technology leverages NESSI-Seq, proprietary library preparation protocol and third generation, native strand sequencing. Native sequencing preserves epigenetic base modifications, allowing researchers to acquire sequence and methylation and hydroxymethylation data in single runs with unparalleled precision and sensitivity. 

This technology is currently being leveraged in research and diagnostic development within diverse therapeutic areas.

Advantages

WBL’s Targeted Methylation Sequencing leverages a proprietary library preparation protocol and third generation, native strand technology capable of directly detecting native methylation and hydroxymethylation modifications. Direct, native detection bypasses the need for bisulfite conversion and PCR amplification, eliminating their associated biases and DNA damage.

In addition to DNA sequence, WBL’s sequencing technology enables the analysis of: 

  • Structural Variants
  • Small Variants (SNPs)
  • Copy Number Variants
    (CNVs, dependent on read length and depth)
  • Short Tandem Repeat Expansions (STRs, dependent on read length and depth)
  • Modified Bases (5mC, 5hmC; also 6mA for bacteria)
  • Phasing Data (dependent on read length and depth)

The Technical Steps

Wasatch BioLab’s Targeted Methylation Sequencing Service leverages proprietary library preparation and hybridization-based enrichment protocols to streamline library prep and the targeted acquisition of native sequence and methylation data. WBL’s NESSI-Seq protocol bypasses the amplification and conversion steps required in traditional methylation analyses, reducing errors and biases in your data.

The Setup Process

1. Identify Your Targets

Select the genomic regions you wish to sequence. WBL’s team is available to assist you in identifying and uploading your target list to your WBL portal.

2. Design Your Probe Set

Schedule a consultation with a WBL project manager to customize your probe set, tailored to meet the needs of your research.

3. Prepare Your Samples

Extract genomic DNA (gDNA) from your sample(s) using any standard commercial kit.

4. Ship Your Samples

Send your extracted samples to WBL for processing (WBL Shipping Instructions).

5. Sequence Samples

WBL will perform targeted sequencing on your samples using proprietary NESSI-Seq technology.

6. Monitor Your Project

Receive real-time updates and track the progress of your sequencing project via your WBL portal.

7. Access Your Data

Upon completion, sequencing and methylation reports will be available for download through your WBL portal.

Richer data with WBL’s Targeted Methylation Sequencing

Tissue of Origin Identification Targeting 1,235 regions (2,000 bp each) with tissue-specific methylation patterns using 4,940 probes resulted in accurate identification and quantification of gDNA from our tissue of interest. Analysis of 2000 randomly selected reads yielded a correlation between predicted and true fractions of a dilution series of R2 = 0.999.

Download Whitepaper
Download Whitepaper

WBL: Your Competitive Edge

Maximize your sequencing output with WBL’s leading technology, streamlined bioinformatics pipelines, and expert guidance. WBL’s team of researchers are pioneers in Oxford Nanopore Technologies sequencing with decades of experience in genomics and bioinformatics research.

Speak To A Specialist
SPEAK TO A SPECIALIST

Robust Data

NGS 3.0 data with fully streamlined bioinformatics pipelines.

Fast Turnarounds

Results within 14-21 days of your sample’s arrival at the lab.

Expert Guidance

Teams of dedicated researchers and project managers to support your research and development.

Full Visibility

Direct access to an online portal for sample information and batch status at each step of the sequencing process.

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